Inoculation technique Agarwood

noculation technique GAHARU Spiral Systems Simple:




1. Inoculation

Inducer Bio Chemistry / Fusarium sp that inoculation to the network tree was actually disease-causing germs. Therefore it is against the agarwood trees produce resin called phytoalexin so germs do not spread to other tree network. Over time, the resin hardens corner angle xylem and phloem vessels - organs that distribute tree lightly browned and fragrant when burned.

Given the kinds of aloes-forming disease isolates vary according to climate and environmental conditions, then the inoculant providers need to do their prospective isolation diseases produce agarwood. The isolation is carried out on natural aloe plant is located in the forest area surrounding the development area. For this purpose, it should be preceded by field observations for studying aspects of aloes that grow naturally and isolate and identify the type of disease from infected trees.

In order to successfully develop an inoculant forming aloes, required a certain technique. For this, the indispensable role of local government agencies or institutions, universities, private entrepreneurs and investors or local area as the perpetrators of inoculant production.

Stages in penginokulasian aloes, materials and equipment needed are:

70% alcohol to sterilize tools and wood drill hole results.

Wood drill with a minimum size of 10 mm, in accordance with trunk diameters greater the diameter the size of the larger drill, drill size commonly used size of 13 mm.
Genset capacity of 450 watts or 900 watts and power drill tool.
Permanent marker to mark the point of the drill.

Masks, scissors and cotton.
Measuring instrument gauge for measuring the circumference rods and drill point distance of one another.
Tweezers and injections according to the size of the drill.

Rubber gloves and inoculants Eaglewood.

Soft wax, plaster or tape, to seal the borehole.

reaching 8-10 cm.

The following reviews inoculation technique using solid and liquid inoculants.

a. Inoculation With Solid inoculants

Make a hole in the trunk of aloes wood by using a drill.

Agarwood tree inoculation technique uses solid inoculant as follows:

Drill hole diameter of about 0.8 to 13 mm. Optimal drilling depth need to be adjusted to the size of the diameter of the rod, usually around 5 cm. Each rod made a lot of holes spaced drill holes approximately 20 cm.

Clean hands perpetrators inoculation with water until clean and rinsed with alcohol prior to the inoculation.

Cover any holes that have been given inoculant for mnghindari entry of water into the hole. The closure of these holes is done with wooden pegs aloes. Closure can be done with the "wax"

Enter a solid inoculation into each hole. The amount of inoculant adjusted to the depth of the hole. As a rule, this income made up pit is filled with inoculant. In order for income to be easy, use a piece of wood or bamboo whose size corresponds to the diameter of the hole.

Workmanship by following the procedures below:

Measure the initial drilling point 1 meter above the ground. Mark with a marker. Then create another drilling point thereon to shift the horizontal direction as far as 15 cm and 15 cm vertical. in the same way to make the next point after being linked to form a spiral line.
Measure circle to get the rod diameter rod. Suppose circle 60 cm rod, calculate its diameter with the formula: circumference Circle = diameter x 3.14. example 60 cm = diameter x 3.14 mean = 60 cm stem diameter: 3,14 = 19.11 cm.

Enter into an injection inoculant with tweezers whose tip is cut, then enter the inoculant into the hole until it is full.
Make a hole as deep as 1/3 trunk diameter at the point of drilling that has been marked with a marker. Example: The depth of the borehole = stem diameter x 1/3 = 19.11 x 1/3 = 6.4 cm.
Clean the drill holes with cotton that has been rinsed with alcohol.

7 (seven) months after injection of grab samples by drilling new holes 5 cm above the previous hole, if the drill results have been black powder or perfume or in accordance with the desired characteristics of the tree can be harvested already if it is not close back in the hole with wax. Signs began maximum results if aloes dried leaves 50% which usually occurs in 1.5 years to 2 years after the injection depending on the size of stem diameter, the larger diameter rod then leaves the longer the drying process.
Close the holes have been filled inoculant with wax so that no contaminants from other microbes. To prevent water from seeping wax surface resealed with tape or duct tape.
Check the success of the injection after 3 months, how open plaster and wax and then peel back a bit of bark, if the stem was colored dark brown victimization means injecting succeed. Cover the hole with wax and plaster.

On the implementation of the agarwood trees penginokulasian this, be aware of the age and the diameter of the trunk. The minimum limit of a tree can be characterized by the inoculation tree starts flowering. Usually the age of the plant is approximately 4-5 years or diameter of the stem is



b. Inoculation With Liquid inoculants

Inoculation technique uses a liquid inoculant as follows:

Perform drilling at the base of a tree trunk with tilt down position. Drilling depth adapted to the diameter of the tree trunk, usually 1/3 diameter rods. While the drill bit used the same size with the IV tube about 0.5 cm. The infusion hose manufacturers usually provided at time of purchase inoculant inoculant. However, if not available, infusion hoses can be supplied by the farmers themselves.

Enter the IV line is on the bottle of liquid inoculant into the hole.

Adjust the amount of flow of the liquid inoculant. Stop infusion if a liquid inoculant flow already out of the hole.


Cover the hose around the edge of the infusion using the "wax".

Repeat settings infuse fluid inflow into the hole every 1-2 days, depending on the state of the fluid in the pit. The setting is done when the flow of holes no longer exist inoculation fluid.

Implement this penginokulasian up inoculant infusion liquid in the bottle is depleted. Penginokulasian repeated with a new inoculation bottle, if there are no signs of physical and physiological death.

2. Maintenance and Fertilization

Fertilizing should be done, especially in the low fertility of land. Fertilizer application can be done twice a year, with a size of 5 kg of fertilizer per tree. Cleaning the planting area also needs to be done in order to avoid the growth of weeds (plants), especially during the rainy season or 4 times a year.





3. Harvest and Post Harvest

Production of pig aloes will be formed after a 3 month treatment. It starts with the changing color of the wood around the injection into a textured brown and hard and smell fragrant. Harvesting can be done starting from the first year after penginokulasian by felling trees. The quality of pig aloes produced is directly proportional to the fertility rate and duration penginokulasian tree. The longer penginokulasian the higher the quality of the pig aloes produced. The pieces of pig aloes is removed from the timber which is not formed into the sapwood. Cleaning eucalyptus from sapwood require workers who have special skills, so it does not degrade the pig classes due to lack of skilled labor. Then do the sorting by class (Super, AB, BC, C1 and C2). To reduce the water content, the pieces of pig aloes dried by drying in the sun. For grade agarwood kemedangan than can be marketed directly can also be distilled to take the oil.